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  JOURNAL "NP" ISSUES

"Nauchnoe Priborostroenie", 2025, Vol. 35, no. 2. ISSN 2312-2951

"NP" 2025 year Vol. 35 no. 2.,   ABSTRACTS

ABSTRACTS, REFERENCES

V. E. Kurochkin1, F. L. Vladimirov1, A. S. Al’dekeeva1,
N. A. Korneva1, M. V. Volkov2, M. S. Shlyakhtin2, D. V. Zhurba2

LASER TECHNOLOGY FOR REMOVING POLYIMIDE
COATING FROM QUARTZ CAPILLARIES

"Nauchnoe priborostroenie", 2025, vol. 35, no. 2, pp. 3—19
 

The article presents the results of a laser method for removing a protective polyimide coating from quartz capillaries in comparison with a thermal method using a gas burner. It has been shown that the use of pulsed ultraviolet laser radiation allows the removal of the protective polyimide coating without the need for additional mechanical processing of the capillaries, heating, the risk of deformation and defectation of the capillaries. Laser technology for removing polyimide coating from quartz capillary allows for high reproducibility of the technological process and is promising for commercial production of capillary arrays.
 

Keywords: laser technology, removing of polyimide coating, quartz capillary

Author affiliation:

1Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia
2JSC "Scientific Industrial Enterprise of Fiber Optics and Laser Equipment" (NPP VOLO),
Saint Petersburg, Russia

 
Contacts: Vladimirov Fedor L'vovich, fedor.vlv3008@gmail.com
Article received by the editorial office on 20.01.2025

Full text (In Russ./In Eng.) >>

REFERENCES

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    0%D1%80%D1%86%D0%B5%D0%B2%D0%BE%D0%
    B5_%D1%81%D1%82%D0%B5%D0%BA%D0%BB%D0%BE
 

V. V. Manoilov , A. G. Borodinov, A. Ya. Loginov,
A. I. Petrov , I. V. Zarutsky , V. E. Kurochkin

CORRECTION OF FLUORESCENT SIGNALS DISTORTED BY OVERLAPPING SPECTRAL
BANDS IN THE "NANOFOR SPS" SEQUENCER

"Nauchnoe priborostroenie", 2025, vol. 35, no. 2, pp. 20—35
 

An important stage in the technology of massively parallel sequencing of nucleic acids is the process of measuring fluorescence signals. The spectral composition of the fluorescence signal carries information about the type of the fluorescence object (nucleotide) included in the DNA sample analyzed by the sequencer. The more nucleotides are correctly recognized during the reading process, the more accurate the result of reading the entire genome will be. The phenomenon of overlapping spectral components of the fluorescence signals of nucleotides A, C, G and T (cross-talk) is one of the negative factors that prevents reliable reading of the genome.
To combat this phenomenon, a color correction algorithm is needed. This algorithm is decisive in determining the type of nucleotide located in these coordinates (cluster) in a given reading cycle. The paper considers a color correction algorithm that allows one to determine a crosstalk matrix using the linear regression method. Based on this matrix, an inverse matrix is determined, the use of which allows one to significantly reduce the influence of spectral band overlap on the accuracy of fluorescence signal measurement evaluation. The algorithm has been tested on experimental data and has shown high reliability of the results.
 

Keywords: nucleic acid sequencing, mathematical data processing, crosstalk phenomenon, crosstalk matrix, linear
regression, least squares method nucleic acid sequencing, mathematical processing and classification of multivariate data, principal component analysis, machine learning

Author affiliation:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Manoilov Vladimir Vladimirovich, manoilov_vv@mail.ru
Article received by the editorial office on 06.09.2024

Full text (In Russ./In Eng.) >>

REFERENCES

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  2. Polnogenomnyi sekvenator Nanofor SPS [Nanofor SPS Full Genome Sequencer]. URL: https://lab.scienceid.net/ catalog/9824/28806/ (accessed 10.12.2023). (In Russ.).
  3. Manoilov V.V., Borodinov A.G., Zarutsky I.V., Pet-rov A.I., Kurochkin V.E. [Algorithms of Processing Fluorescence Signals for Mass Parallel Sequencing of Nucleic Acids]. Trudy SPIIRAN [SPIIRAS Proceedings], 2019, vol. 18, no. 4, pp. 1010—1036. (In Russ.). DOI: 10.15622/sp.2019.18.4
  4. Borodinov A.G., Manoilov V.V., Petrov A.I., Zarutsky I.V., Kurochkin V.E., Saraev A.S. [Machine Learning in Base-Calling for Next-Generation Sequencing Methods]. Informatika i avtomatizatsiya [Informatics and Automation], 2022, vol. 21, no. 3, pp. 572—603. DOI: 10.15622/ia.21.3.5 (In Russ.).
  5. Manoilov V.V., Borodinov A.G., Saraev A.S. Pet-rov A.I., Zarutsky I.V., Kurochkin V.E. [Image processing algorithms in the DNA Sequencer "Nanofor SPS"]. Zhurnal tekhnicheskoi fiziki [Journal of Technical Physics], 2022, vol. 92, no. 7, pp. 985—992. DOI: 10.21883/JTF.2022.07.52655.318-2 (In Russ.).
  6. Li L., Speed T.P. An estimate of the crosstalk matrix in four-dye fluorescence-based DNA sequencing. Electrophoresis, 1999, vol. 20, no. 7, pp. 1433—1442. DOI: 10.1002/(SICI)1522-2683(19990601)20:7<1433::AID-ELPS1433>3.0.CO;2-0
  7. Whiteford N., Skelly T., Curtis Ch., Ritchie M.E., et al. Swift: primary data analysis for the Illumina Solexa sequencing platform. Bioinformatics, 2009, vol. 25, no. 17, pp. 2194—2199. DOI: 10.1093/bioinformatics/btp383
  8. Ye Ch., Hsiao Ch., Bravo H.C. BlindCall: ultra-fast base-calling of high-throughput sequencing data by blind deconvolution. Bioinformatics, 2014, vol. 30, no. 9, pp. 1214—1219. DOI: 10.1093/bioinformatics/btu010
  9. Cacho A. Base-Calling of High-Throughput Sequencing Data Using a Random Effects Mixture Model (A Dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy UNIVERSITY OF CALIFORNIA RIVERSIDE). URL: https://escholarship.org
  10. Chargaff E. Chargaff's rules. URL: https://en.wikipedia.org/wiki/Chargaff%27s_rules
  11. Manoilov V.V., Borodinov A.G., Saraev A.S., Petrov A.I., Zarutsky I.V., Kurochkin V.E. [Machine learning algorithm for the construction of a nucleotide sequence in the "Nanofor SPS" sequencer using the principal component analysis]. Nauchnoe Priborostroenie [Scientific Instrumentation], 2023, vol. 33, no. 2, pp. 35—48. URL: http://iairas.ru/mag/2023/abst2.php#abst4 (In Russ.).
 

E. V. Voloschenko, S. P. Tarasov, A. P. Voloschenko

PARAMETRIC RADIATION ANTENNA
FOR HYDROACOUSTIC MONITORING OF THE WATER
BODY SURFACE: ANALYSIS OF APPLICATION POSSIBILITIES
IN WAVE MEASURING EQUIPMENT (REVIEW)

"Nauchnoe priborostroenie", 2025, vol. 35, no. 2, pp. 36—56
 

The article considers the use of a parametric radiating antenna (PRA) as a source of a low-frequency ultrasonic signal with tunable characteristics for wave measuring equipment used for hydroacoustic monitoring of the water surface of a coastal area, for example, in remote assessment of the degree of waves when measuring hydroconditions. The results of model experiments on studying the patterns of ultrasonic field scattering during underwater irradiation of both smooth and wind-disturbed water—air interfaces are discussed. The data analysis allowed us to refine the PRA model as a tool for echo detection of objects in shallow water near the acoustically soft water—air interface, employing the imaginary source method. An informative feature is proposed and the basics of the method for measuring the parameters of a rough sea surface using nonlinear acoustics effects are discussed, for example, for the mode of broadband irradiation with ultrasonic signals from the bottom.
 

Keywords: parametric radiating antenna, nonlinear acoustics, scattering of ultrasonic waves on a rough water—air interface

Author affiliations:

Southern Federal University, Taganrog, Russia

 
Contacts: Voloshchenko Elizaveta Vadimovna, voloshchenko.liza@mail.ru
Article received by the editorial office on 14.03.2025

Full text (In Russ./In Eng.) >>

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    URL: https://iairas.ru/mag/2024/abst2.php#abst7 (In Russ.).
 

E. E. Maiorov1, V. P. Pushkina1, R. B. Guliyev1, A. V. Dagaev2

EXPERIMENTAL STUDY OF INTERFERENCE SETUP USING WHITE
LIGHT SOURCE FOR SURFACE MICRORELIEF MONITORING

"Nauchnoe priborostroenie", 2025, vol. 35, no. 2, pp. 57—66
 

The study highlights the problem of metrology of non-smooth surfaces and the identification of the technical characteristics of the developed interferometric installation, where a white light diode was used as a radiation source. Obtaining high-precision information about the geometry of an optical or non-smooth surface is a signi­ficant problem for metrological support, and interference devices and complexes using light sources that block the laser mode are able to provide the researcher with such information. The metrological parameters of the experimental setup are investigated: the range, measurement error, and amplitude of the interference signal. The experimental results obtained are presented in the form of technical specifications.
 

Keywords: light wavelength, contrast of the interference field, coherence length, measurement error, measurement range, amplitude of the interference signal, diameter of the light spot

Author affiliations:

1Saint Petersburg state university of aerospace instrumentation (GUAP), Saint Petersburg, Russia
2Ivangorod Humanitarian and Technical Institute (branch) of the Federal State Autonomous
Educational Institution of Higher Education "St. Petersburg State University of Aerospace Instrumentation", Ivangorod, Russia

 
Contacts: Maiorov Evgeniy Evgen'evich, majorov_ee@mail.ru
Article received by the editorial office on 16.04.2025

Full text (In Russ./In Eng.) >>

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  7. Maiorov E.E., Kostin G.A., Chernyak T.A., Baranov N.E. [Use of radiation with low time coherence to study the surface of slotted flaps on the aircraft wing]. Izvestiya Tul'skogo gosudarstvennogo universiteta. Technicheskie nauki [News of Tula State University. Technical sciences], 2023, iss. 7, pp. 577—581. (In Russ.).
  8. Maiorov E.E., Aref'ev A.V., Kurlov V.V., Pushkina V.P., Borodyanskiy Yu.M., Tayurskaya I.S. [Experimental study of cosmetic oils with low-coherence interference radar]. Izvestiya Tul'skogo gosudarstvennogo universiteta. Technicheskie nauki [News of Tula State University. Technical sciences], 2023, iss. 7, pp. 585—590. (In Russ.).
  9. Maiorov E.E., Aref'ev A.V., Guliev R.B., Pushkina V.P., Dagaev A.V. [Interference method in the study of the surface of polymer microchannels and quartz microfluidic chips]. Pribory [Measuring instrument], 2024, no. 2, pp. 28—32. (In Russ.).
  10. Maiorov E.E., Kostin G.A., Kurlov V.V., Baranov N.E. [Study of the dynamic surface of an aircraft by the interference method]. Izvestiya vysshikh uchebnykh zavedeniy. Priborostroenie [Journal of Instrument Engineering], 2024, vol. 67, no. 8, pp. 722—726. DOI: 10.17586/0021-3454-2024-67-8-722-726 (In Russ.).
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D. V. Lisin

ARCHITECTURE OF THE POWER SUPPLY SYSTEM
FOR SPACE EXPERIMENTAL COMPLEXES BASED
ON LITHIUM-ION BATTERIES

"Nauchnoe priborostroenie", 2025, vol. 35, no. 2, pp. 67—77
 

The architecture of building a highly reliable power supply system based on lithium-ion batteries, designed for conducting scientific experiments on spacecraft board, is considered. The proposed approach is based on many years of experience in creating experimental scientific equipment complexes and makes it possible to overcome a number of fundamental disadvantages of lithium-ion batteries, opening up the possibility of using their undeniable advantages during the creation of new promising instrument complexes for various purposes in Earth orbit. Particular attention is paid to considering issues specific to the application area, such as the initial power-up of the complex, its ground testing, and ensuring reliable operation.
 

Keywords: power supply system, lithium-ion battery, space experiment, backup management

Author affiliations:

Pushkov Institute of terrestrial magnetism, ionosphere and radio wave propagation
(IZMIRAN), Troitsk, Moscow, Russia

 
Contacts: Dmitrij Valer'evich Lisin , lisindv@izmiran.ru
Article received by the editorial office on 05.03.2025

Full text (In Russ./In Eng.) >>

REFERENCES

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    jelektronika     [Power electronics], 2021, vol. 91, no. 4, pp. 62—65. (In Russ.). URL: https://power-e.ru/hit/kontrol-poelementnyh-napryazhenij/
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N. A. Vodichev, D. A. Gavrilov, A. V. Leus, V. A. Efremov, I. V. Kholodnyak, V. A. Zuev,
M. M. Parshikov, A. Yu. Troegubov, N. V. Gershtein, V. E. Laukhin, A. E. Antipov

ALGORITHM FOR CALCULATING THE POSITION
AND SPEED OF OBJECTS ON A PLANE USING DATA
FROM A MONOCULAR CAMERA

"Nauchnoe Priborostroenie", 2025, vol. 35, no. 2, pp. 78—96
 

This article presents an algorithm for the automatic calculation of a homography matrix, intended for estimating the speed of forklifts in warehouse conditions. The proposed algorithm uses video streams from surveillance cameras to transform object coordinates from a perspective image into a metric space, enabling the determination of forklift positions in real measurement units. The algorithm implements automatic calibration based on the recognition of pallets with known dimensions and the use of camera characteristics. An automatic parameter selection algorithm is proposed for the monocular system in cases where these parameters are not known in advance. An experiment using real video recordings demonstrates the applicability of the proposed algorithm for monitoring logistics processes and improving the efficiency of warehouse transport management. The experiment showed that when calculating average forklift speed, 98% of the obtained values fall within an error margin of ±1 km/h.
 

Keywords: speed calculation, computer vision, neural networks, homography matrix

Author affiliations:

Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia

 
Contacts: Vodichev Nikita Alekseevich, vodichev.na@phystech.edu
Article received by the editorial office on 20.03.2025

Full text (In Russ./In Eng.) >>

REFERENCES

  1. Vodichev N. et al. Real-time distance estimation algorithm for objects in warehouse based on monocular camera data for an autonomous unmanned forklift. The European Physical Journal Special Topics. Springer, 2025. P. 1—13. DOI: 10.1140/epjs/s11734-024-01452-4
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B. P. Sharfarets

REGARDING THE PROBLEM OF SOLVING THE POISSON—BOLZMANN
EQUATION WHEN THE DEBYE—HUCKEL APPROXIMATION CONDITIONS
IN ELECTROKINETIC PROCESSES ARE NOT MET

"Nauchnoe Priborostroenie", 2025, vol. 35, no. 2, pp. 97—104
 

The paper presents a set of mathematical models used in the numerical modeling of electrokinetic problems, namely, mathematical models for calculating electrokinetic processes during the study of the motion of an incompressible viscous fluid in a circular capillary. The corresponding hydrodynamic equations are given. The Debye—Huckel approximation in electroosmotic processes is considered when solving the Poisson—Boltzmann equation, and methods for solving it in cases where the Debye—Huckel approximation is invalid are given. This allows us to significantly expand the range of zeta potential values at which the Poisson-Boltzmann equation can be solved.
 

Keywords: electroosmosis, viscous incompressible fluid, Navier—Stokes equations system, Debye—Huckel approximation, solution of Poisson—Boltzmann equation in case of invalidity of Debye—Huckel approximation

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Sharfarets Boris Pinkusovich, sharb@mail.ru
Article received by the editorial office on 07.03.2025

Full text (In Russ./In Eng.) >>

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