Instrument catalog

CAPILLARY LIQUID ELECTROCHROMATOGRAPH NANOFOR 04

Universal instrument for analyzing compound biological solutions:

•  peptides and proteins;

•  nucleic acids ;

•  complex biopolymers;

•  low-molecular biologically active substances;

•  synthetic medicines, etc.

NANOFOR 04 is the instrument that realizes capabilities of two techniques : highly efficient liquid chromatography and capillary electrophoresis.

Those techniques are easy in application and efficient; they ensure high reproducibility and accuracy of analysis at extremely small sample sizes (about 2 nl) and low cost of consumables.



Flexible configurations of the NANOFOR 04 electrochromatograph allows one to use either monolithic capillary columns or conventional capillaries, and also to chose the detector type: spectrophotometric or fluorometric.

 

Peculiar features

•  Combination of capabilities of two separation techniques : highly efficient liquid chromatography and capillary electrophoresis.

•  Availability of two detectors (spectrophotometric and fluorometric) mounted in the same casing.

•  Ten-fold increase in the analysis sensitivity due to Z-shaped tray.

•  Fast programmable change of the high-voltage power supply polarity (1 second) in order to concentrate the sample within the capillary.

Remote access to the analysis results through the Internet or local network

 


Basic performance characteristics

LIF detector

Sensitivity threshold with respect to a typical fluorescein sample, M, maximum

 

10 -11

Operating laser wavelength of the detector, nm

 

473

CF detector

Sensitivity threshold with respect to benzoic acid, mg/dm 3 , maximum

0.1

Operating wavelength*, nm

 

214, 254, 280

High voltage power supply

High-voltage setup range, kV

1-30

Reduced accuracy of the high-voltage setup, %, maximum

1

Possibility of changing the polarity in the process of analysis

 

yes

General characteristics

Number of test tubes with samples and buffer solutions

16

Method of sample introduction: electrokinetically or by pressure

yes

Dimensions, mm

500x400x500

* Operating wavelengths depend on the types of easily replaceable light filters and vary within the 200 to 600 nm range.

 

Preferred application

Medical diagnostics, ecology, chemistry, biological technologies, criminalistics and urgent forensic medical examination, food analysis, pharmacology.

 

 

ANALYSIS OF THE BLOOD SERUM BY CAPILLARY ELECTROPHORESIS
WITH THE NANOFOR 04 INSTRUMENT

1 g - globulin (15.35 %); 2 - b - globulin (8.15 %); 3 - a 2- globulin (9.3 %);
4 -a1 - globulin (4.9 %); 5 albumin (60.5 %); 6 low-molecular fraction (1.8 %).

 

 


Legal protection

RF Patent No. 2182329 "Fluorometric detector" dated May 10, 2002 .

RF Patent No. 2189038 "Spectrometric detector for capillary electrophoresis and capillary liquid chromatography" dated September 10, 2002 .

 

 

Manufacter

Institute for Analytical Instrumentation RAS.

 

190103 Russia,St-Petersburg, Rizhsky pr., 26 ; t.: (812) 2518600, fax: (812) 2517038, mail: iap@ianin.spb.su