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  JOURNAL "NP" ISSUES

"Nauchnoe Priborostroenie", 2016, Vol. 26, no. 1 ISSN 2312-2951, DOI: 10.18358/np-26-1-0596

"NP" 2016 year Vol. 26 no. 1,   ABSTRACTS

ABSTRACTS, REFERENCES

K. I. Belousov1, A. S. Bukatin2,3, V. I. Chubinskiy-Nadezhdin4, V. Y. Vasilyeva4,
Y. A. Negulyaev4,5, A. A. Evstrapov1,2,3, I. V. Kukhtevich1,2

MICROFLUIDIC DEVICE WITH Y-SHAPED DESIGN FOR STUDY OF CELL MIGRATION IN CONCENTRATION GRADIENT OF CHEMOATTRACTANTS

"Nauchnoe priborostroenie", 2016, vol. 26, no. 1, pp. 3—10.
doi: 10.18358/np-26-1-i310
 

Results of a development of microfluidic device for a study of cell migration under an influence of chemoattractant are discussed. Numerical simulation of chemoattractant distribution in various designs of microfluidic chip and estimations of the cells’ interaction with the liquid flow are conducted. Microfluidic chip design is chosen based on the simulation results. Microfluidic devices for experimental research are made by a soft lithography method in polydimethylsiloxane. The results of experiments carried out using CHO and HepG2 cells confirm the efficiency of the microfluidic chip and proposed approach.
 

Keywords: microfluidics, microfluidic device, cell migration, concentration gradient, computer simulation

Author affiliations:

1ITMO University, Saint-Petersburg, Russia
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg, Russia
3Saint-Petersburg National Research Academic University RAS, Russia
4Institute of Cytology RAS, Saint-Petersburg, Russia
5Peter the Great Saint-Petersburg Polytechnic University, Russia

 
Contacts: Belousov Kirill Il'ich, belousov_k.i@mail.ru
Article received in edition: 23.12.2015
Full text (In Russ.) >>

REFERENCES

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  10. Kim S., Kim H.J., Jeon N. Biological applications of microfluidic gradient devices. Integrative Biology, 2010, vol. 2, pp. 584—603. Doi: 10.1039/c0ib00055h.
  11. Kukhtevich I.V., Belousov K.I., Bukatin A.S., Evstrapov A.A. [Designs of microfluidic devices for cell migration study in chemical gradients (review)]. Nauchnoe Priborostroenie [Science Instrumentation], 2015, vol. 25, no. 1, pp. 3—16. Doi: 10.18358/np-25-1-i316 (In Russ.).
  12. Yamamoto A., Mishima S., Maruyama N., Sumita M. Quantitative evaluation of cell attachment to glass, polystyrene, and fibronectin- or collagen-coated polystyrene by measurement of cell adhesive shear force and cell detachment energy. Journal of Biomedical Materials Research, 2000, vol. 50, no. 2, pp. 114—124.
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E. N. Chernova1, Y. V. Russkikh1, E. P. Podolskaya1,2, Z. A. Zhakovskaya1

DETERMINATION OF MICROCYSTINS AND ANATOXIN-A USING LIQUID CHROMATO-MASS-SPECTROMETRY OF UNIT RESOLUTION

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 11—25.
doi: 10.18358/np-26-1-i1125
 

We proposed the method for microcystins and anatoxin-a detection using tandem mass spectrometry of unit resolution. The method involves a unique fragment approach.
Diagnostic ions are suggested, and tandem spectra and fragmentation pattern of 9 most widespread microcystins and anatoxin-a obtained using LC/MS/MS under ion trap conditions were described. The approach used in this work is capable to carry out very reliable identification of known microcystins and to state the structural variants when standards are not available. Based on the fragment mass spectra and systematic literature data suggested a new classification of ion products microcystins, greatly facilitates the process of identifying them, and allows for a set of diagnostic fragments to determine the structure of different variants of MS without using standard compounds. The method of the determination of microcystins and anatoxin-a in the environmental samples and drinking water using tandem mass-spectrometry using the ion trap of unit resolution was described.
 

Keywords: microcystins, anatoxin-a, LC/MS/MS of unit resolution, diagnostic ion-products, identification

Author affiliations:

1 Saint-Petersburg Scientific-Research Centre for Ecological Safety RAS (SRCES RAS), Russia
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg , Russia

 
Contacts: Chernova Ekaterina Nikolaevna, s3561389@yandex.ru
Article received in edition: 16.12.2015
Full text (In Russ.) >>

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L. N. Gall1, S. I. Maximov1, T. S. Skuridina1, N. R. Gall1,2

LOW FREQUENCY INDUCTIVE DIELCOMETRY – INFORMATIVE METHOD FOR THE STUDY OF THE STRUCTURING OF WATER IN AQUEOUS SOLUTIONS

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 26—33.
doi: 10.18358/np-26-1-i2633
 

Possibility to study inner water structure using frequency dependence of dielectric loss tangent by low-frequency inductive dielcometry has been considered. Experimental setup at the range 40—800 kHz was based on a standard Q-meter with specialized inductance coils. Features of the method and setup have been demonstrated using bi-distilled water, and solutions of non-organic electrolytes and biologically active molecules with concentrations varied in a wide range.
It has been shown that bi-distillate water presents decreased frequency dependences of dielectric loss tangent; one should use fresh samples to provide good reproducibility. LiNO3 solutions shows strong and non-monotonic dispersion of dielectric loss tangent for the concentration range of 10-4—10-6M; this results in formation of discrete spectra, strongly variable with concentration within the range. At a concentration < 10-6 M, lithium salts present curves similar to those for bi-distilate. On the contrary, bio-active molecular solutions, like potassium phenozan, show nearly similar dispersion spectra for a very wide concentration range at least from 10-5 to 10-16 M.
We propose to interpret the low frequency range of the dielectric loss tangent spectrum as a result of inner water structure originated in contact with the ions and organic molecules.
 

Keywords: strukturing of water, electrolytes, radiospectroscopy, dielcometry

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint-Petersburg, Russia
2Ioffe Physical Technical Institute of the RAS , Saint-Petersburg, Russia

 
Contacts: Gall Lidiya Nikolaevna, lngall@yandex.ru
Article received in edition: 24.11.2015
Full text (In Russ.) >>

REFERENCES

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D. A. Belov1, D. G. Petrov1, Yu. V. Belov1, N. N. Knyaz'kov1, I. G. Kiselev2

EXPERIMENTAL DETERMINATION OF POLYMERASE CHAIN REACTION AMPLIFICATION PARAMETERS OF NUCLEIC ACID ANALIZER

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 34–40.
doi: 10.18358/np-26-i3440
 

The purpose of this article is to identify the conditions that allow to achieve the required test results in minimum time. The method of determination of the real-time polymerase chain reaction (RT-PCR) amplification basic parameters (PCR efficiency and the thermal cycle duration) was suggested. An example of the threshold cycles measuring and calculation of the PCR efficiency of test samples with fluorescent dyes FAM and ROX using breeding techniques. The PCR efficiency measuring error and PCR efficiency dependence on the temperature cycle duration were determined. As a result of the experiments and calculations, the temperature mode with the maximum PCR efficiency closed to the most achievable value was determined. The most effective temperature conditions selection and the cycle time reducing can significantly cut down on the performance of the analysis (it is decreased by 19 % in the experiment).
 

Keywords: real-time PCR, DNA, threshold cycle, PCR efficiency, thermal cycle duration

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint-Petersburg , Russia
2Petersburg State Transport University, Russia

 
Contacts: Belov Yuriy Vasil'evich, bel3838@mail.ru
Article received in edition: 30.01.2016
Full text (In Russ.) >>

REFERENCES

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M. M. Ignatchik1, Y. S. Posmitnaya1,2, A. A. Evstrapov1,2,3

STUDY OF MODIFICATION WETTING PROPERTIES OF THE SURFACE OF POLYDIMETHYLSILOXANE AND THE MICROCHANNEL AFTER EXPOSURE BY HIGH-FREQUENCY PLASMA IN OXYGEN

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 41—46.
doi: 10.18358/np-26-1-i4146
 

Soft lithography is a general technique for micro patterns fabrication in polydimethylsiloxane (PDMS). The surface of PDMS should be treated by physical and/or chemical methods depending on the end goal of modification. An important factor to ensure reproducible measurements on microfluidic chips is the stability of the obtained properties of treatment surfaces in time. We have investigated the change of wetting properties of the microchannel and the open surface of PDMS after plasma treatment depending on storage time. The estimation of the contact angle in the microchannel allow to evaluate the efficiency of processing or modification of the working surface. The possibility of determining the relationship between the contact angle on the substrate of PDMS and the meniscus of the liquid in the channel is discussed.
 

Keywords: microfluidic chip, contact angle, plasma treatment, polydimethylsiloxane

Author affiliations:

1ITMO University, Saint-Petersburg , Russia
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg , Russia
3Saint-Petersburg National Research Academic University, Russian Academy of Sciences, Russia

 
Contacts: Ignatchik Makar Michaylovich, marchibald93@gmail.com
Article received in edition: 11.01.2016
Full text (In Russ.) >>

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A. V. Grebenyuk1, S. M. Irkaev1, V. V. Panchuk1,2, V. G. Semenov1,2

AB INITIO CALCULATION OF OPTIMUM ABSORBER THICKNESS IN MÖSSBAUER SPECTROSCOPY

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 47—53.
doi: 10.18358/np-26-1-i4753
 

The aim of study is to optimize the Mossbauer experiment parameters. The main criterion for optimization is the quality of the experimental spectrum SNR, which is determined by the signal to noise ratio S / N. One of the most important parameters affecting on the quality of the spectrum is the absorber thickness.
The introduction of the article is devoted to the theoretical consideration of the influence of the absorber thickness on the quality of spectrum. Analysis of the theoretical dependence of the shape of the spectrum allowed us to propose the technique of choosing the optimal thickness, based on the ab initio calculation of the transmission integral.
On the basis of the proposed technique were calculated the optimum thickness for the model samples, which have spectra with different hyperfine structure and the different contributions of non-resonant absorption. When selecting the chemical composition of model samples the main attention was paid to the influence of matrix effects. These effects are directly depend from the ratio of resonant to nonresonant atoms in the absorber.
The experimental part of the project was dedicated to test the theoretically predicted optimum absorber thickness with real ones. We measured the number of Mössbauer spectra of synthesized samples with different thicknesses. The results of these measurements were plotted as dependence of the experimental spectrum quality with the absorber thickness. Experimentally found optimum thickness values coincided with the theoretically calculated one. It was shown that the optimum thickness depends strongly on the matrix in which the resonance isotopes are placed. In the case of heavy matrix with a high influence of non-resonant absorption the optimum thickness of the absorber shifts towards lower values. The presence of background only reduces the absolute values of the signal / noise ratio and does not affect on the position of the maximum.
 

Keywords: ab initio calculation, Mössbauer spectroscopy, Lamb–Mössbauer factor, optimum absorber thickness, quality of experimental spectrum

Author affiliations:

1Institute for Analytical Instrumentation RAS, RF
2Sankt-Petersburg State University, RF

 
Contacts: Irkaev Sobir Mulloevich, sobir_irkaev@mail.ru
Article received in edition: 2.12.2015
Full text (In Russ.) >>

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A. I. Zhernovoy, S. V. Diachenko

COMPARE OF SIZES END OF MAGNETIC MOMENTS NANOPARTICLES OF A MAGNETIT IN A COLLOID SOLUTION AND IN A POWDER, PREPARED BY A CEMICAL SEDYMENTATION

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 54—57.
doi: 10.18358/np-26-1-i5457
 

The magnetization curves of a powder and of a colloidal solution be received to define a sizes and a magnetic moments of nanoparticles. Size of a powder nanoparticle prove to be equal 2.88 of size a nanoparticle of colloidal solution, and a magnetic moment of a powder nanoparticle prove to be equal 1.44 of that colloidal solution. That`s why, powder nanoparticle content 2—3 nanoparticles of colloid solution with a nonparallel magnetic moments.
 

Keywords: magnetite powder, colloidal solution of nanoparticles of magnetite, nanoparticle size, magnetic moment of a nanoparticle

Author affiliations:

Saint-Petersburg State Institute of Technology (Technical University)

 
Contacts: Zhernovoy Aleksandr Ivanovich, azhspb@rambler.ru
Article received in edition: 21.12.2015
Full text (In Russ.) >>

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    URL: http://213.170.69.26/mag/2009/full3/Art8.pdf (In Russ.) .
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Ya. A. Fofanov, B. V. Bardin

ON THE POLARIZATION RESPONSES OF THE OBJECTS WITH A SMALL OPTICAL ANISOTROPY

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 58—61.
doi: 10.18358/np-26-1-i5861
 

The polarization-optical methods are effective means of studying of the most various objects and systems. Materials and elements with high optical and structural homogeneity, the ordered substances and materials, resonant and nonresonant ensembles of the point scatterers, and so on may be the subject of research and technological monitoring. Nevertheless, the prospects for further development and application of the methods are limited in some cases due to the lack of sufficiently complete picture of their capabilities. Some features of polarization responses of the objects with small optical anisotropy are considered in this work. It is shown that these responses possess properties of linearity and additivity, that are important for practice. Principle possibility of the description of magnetization in terms of orientational order is discussed.
This work is supported by RFBR, grant ¹ 15-02-08703.
 

Keywords: laser, polarization-optical analysis, magnetooptics, optoelectronics, optical materials

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint-Petersburg, Russia

 
Contacts: Fofanov Yakov Andreevich, yakinvest@yandex.ru
Article received in edition: 7.12.2015
Full text (In Russ.) >>

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D. V. Dvortsov, V. A. Parfenov

USING LASER DIODES FEATURES FOR REGISTRATION ABSORPTION LINES OF IODINE

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 62—67.
doi: 10.18358/np-26-1-i6267
 

The problems arising from the using of red FP (Fabry–Perot) laser diodes for registration of Doppler broadened absorption lines of iodine (127I2) are considered in the paper. They represent the features of single-frequency mode regime of generation of these lasers. The influence of temperature and injection current on single-frequency operation range is one of them. Another problem is associated with a considerable slope of the dependence of the generation frequency versus temperature and current. The boundaries of single longitudinal mode regime range were defined by registration of spectrum and intensity of modes by means of scanning interferometer. To make the registration of Iodine absorption lines easier we used frequency tuning with simultaneous frequency scanning by modulation of laser current and discrete temperature tuning. Obtained results can be used for registration of another gas absorption lines and stabilization of frequency of laser diodes radiation.
 

Keywords: laser diode, semiconductor laser, single-frequency mode of operation, single longitudinal mode regime, frequency tuning, absorption line of iodine

Author affiliations:

Peter the Great Saint-Petersburg Polytechnic University, Russia

 
Contacts: Parfenov Vladimir Aleksandrovich, ppparfen@mail.ru
Article received in edition: 22.12.2015
Full text (In Russ.) >>

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V. V. Gorbatsky1, V. A. Elokhin2, V. I. Nikolaev2, T. D. Ershov2, A. Yu. Elizarov3

RESEARCH DISSOLVED IN SEA WATER BY MEANS OF GAS MASS SPECTROMETER WITH MEMBRANE SEPARATOR INTERFACE

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 68—76.
doi: 10.18358/np-26-1-i6876
 

Underwater mass spectrometer has been used to detect and measure the concentration of oil in the sea water by measuring the concentrations of the three hydrocarbons: benzene, toluene and xylene in the area of specialized sea oil port of Primorsk in the Gulf of Finland in situ. Sample input seawater into the quadrupole mass spectrometer was monitored through the membrane interface with a silicone membrane. The resulting mass spectra demonstrated the ability to measure the concentration of oil in the sea water and determine the type of oil without sample preparation. We investigated the sensitivity of the mass spectrometer at different temperatures of sea water and the suspension of oil droplets of different diameters. It is shown that the use of a mass spectrometer with membrane separator interface allows to measure the concentration of hydrocarbons with high precision, which is currently not supported in commercially available monitors.
 

Keywords: mass spectrometer, pollution, express analysis, membrane ecology hydrocarbons, shelf, oil refining

Author affiliations:

1Krylov State Research Center, Saint-Petersburg, Russia
2Scientific Instruments, Saint-Petersburg, Russia
3Ioffe Physical Technical Institute of the RAS, Saint-Petersburg, Russia

 
Contacts: Elizarov Andrei Yurievich, a.elizarov@mail.ioffe.ru
Article received in edition: 24.11.2015
Full text (In Russ.) >>

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N. N. Knyaz'kov, B. P. Sharfarets

ACOUSTICS OF POROUS-ELASTIC FLUID SATURATED MEDIUM
(AN OVERVIEW OF THE BIOT THEORY)

"Nauchnoe Priborostroenie", 2016, vol. 26, no. 1, pp. 77—84.
doi: 10.18358/np-26-1-i7784
 

The overview provides the basic elements of the Biot theory for acoustics of porous fluid saturated media. The theory is valid and for actually porous and for granular structures. The coupled equations of motion in frequency-spatial domain are given, which allow to calculate the various fields describing the dynamics of the harmonic problems of acoustics of porous media. All the data, allowing to put and solve the corresponding boundary value problems are given. Held fairly comprehensive bibliographic review, which allows if necessary yourself to add not covered in the review elements of the Biot theory. The theory allows to solve, among them various problems of analytical instrumentation.
 

Keywords: saturated porous medium, saturated granular medium, Biot theory, coupled equations, frequency-spatial representation

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint-Petersburg , Russia

 
Contacts: Sharfarets Boris Pinkusovich, sharb@mail.ru
Article received in edition: 11.12.2015
Full text (In Russ.) >>

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