Instrument catalog

ANK-16 AND ANK-32 DEVICES FOR ANALYSIS OF DNA AND RNA BASED ON REAL-TIME PCR

Function

The devices are designed for detection and quantitative determination of specific DNA (RNA) in the tested samples in a wide dynamic range up to 109 copies.

The devices come in two versions: ANK-16 and ANK-32 for analysis of 16 and 32 samples, respec-tively. They are suitable both for research and routine analyses.

The ANK device consists of a thermal unit, optical unit, and software.

The thermal unit provides an exponential increase of the PCR product (amplicon) amount in the pres-ence of the target DNA (RNA) in the sample.

The optical unit measures the sample fluorescence signal proportional to the amplicon amount in the reaction mixture. Five independent reactions in a given sample can be simultaneously observed due to five–color measurement of the fluorescence signal.

The device software supports setting and control of PCR and fluorescence measurement conditions as well as PCR data processing and output in a specified form.

 

Main technical characteristics

Number of wells for vials

16 or 32

Types and volume of test tubes, mL

Standard, with transparent dome lid, 0.2

Dynamic temperature control of vials with samples according to a prescribed cyclogram in the range, ° Ñ :


4–99

Well–to–well temperature variation, ° Ñ

± 0. 15

Absolute well temperature error in the 40 to 95 ° Ñ range, ° Ñ

± 0. 1

Vial heating (cooling) rate, ° Ñ /s, no less than

1.5

Controlled amplifier lid heating, ° Ñ

95– 110

Lid temperature maintenance accuracy, ° Ñ

± 1

Light source

Halogen lamp

Light detector

PMT

Excitation wavelength , nm

490, 530, 580 , 630, 680

Detection wavelength , nm

520, 550, 610, 670, 710

Sensitivity in each channel for respective fluorescent dyes (FAM, R6G, ROX and Cy5), M, no less than

2.5 • 10-9

Power consumption , W

350

Overall dimensions , mm

340 x 400 x 420

Weight , kg

26

Warranty service period, years

2

 

Application

Medicine, sanitary and epidemiologic control, agriculture, criminalistics, biotechnology, basic research in the field of nucleic acid analysis.

 

Features

•  DNA/RNA quantitation from a single to10 –9 copies

•  up to five-colours fluorescence detection, possibility of detecting a great number of fluorescent dyes – SYBR Green I, FAM, R6G, HEX, JOE, ROX, TAMRA, Cy5, Cy5.5 and other

•  possibility of multiplex PCR design

•  high sensitivity of all detection channels, which allows working with down to 0,5 pico-mole probes per reaction

•  user-friendly, convenient software, modern algorithms of threshold cycles calculation and ability to choose variants of data analysis

•  reduction of contamination risks, time saving, simplification of PCR-laboratory organization

 

KINETIC CURVES OF AMPLIFICATION OF HUMAN IgG GENE DNA FRAGMENT

Ïîäïèñü: High accuracy and fine precision of measurement results. The spread in values of threshold cycles for 32 equal samples amounts to ±0.11 Ïîäïèñü: Possibility of identifying small differences in amounts of original DNA (in this case the amount of DNA in samples is 5 and 10 thousand copies)

 

TEST-SYSTEM FOR GMO DNA DETECTION (35S-PROMOTER)

 

Wide dynamic range of DNA quantitation (from single copies to 109 copies)

 

TEST-SYSTEM FOR M. tuberculosis DNA DETECTION

Possibility of multiplex PCR design.
The first color channel – detection of DNA fragment IS6110; the second channel – internal positive control; the third channel – detection of DNA fragment of gene senX3-regX3 of M. Tuberculosis

 

 

Certification

Registration of Health and Social Development Ministry No. ÔÑ 022 à 2005/2163-05 of August 23, 2005 .

Protected by RF Patent issued 10 Aug, 2007

 

Developer

Institute for Analytical Instrumentation RAS – optical unit and overall adjustment.

Bauman Moscow State Technical University – high-precision programmable thermal unit.

SYNTOL Close Joint–Stock Company – chemical reagents for RT–PCR.

 

Delivery kit

The devices come complete with software, methods package, calibration kit, primers, dyes and other re-agents (from SYNTOL).

Optional supply:

Cyclotemp-901 centrifuge for protein and DNA samples preparation (developed by N.E. Bauman Moscow State Technical University ).

190103 Russia,St.Petersburg, Rizhsky pr., 26 ; t.: (812) 2518600, fax: (812) 2517038, mail: iap@ianin.spb.su