HIGH  TECHNOLOGIES  IN  MICROBIOSEPARATION
(A review based on the publication in "Electrophoresis", 2004)
B. G. Belenkii

Institute for Analytical Instrumentation RAS, Saint-Petersburg


 
 

    This paper is an extended version of the Russian translation from English of the review article "Capillary Electrochromatography of Peptides and Proteins" by Yan Li et al. ("Electrophoresis", 2004, 25, 2242-2256). This is an overview of recent advances in bioanalysis using capillary electrochromatography (CEC) for separation of proteins and peptides. Basic principles of CEC are briefly described. Much attention is given to the development of new stationary phases and new column designs, their advantages and disadvantages. New CEC column technologies, including monolithic ones, recently developed to achieve high speed and efficiency of separation are considered. Various analytical CEC platforms are introduced, namely: pressure assisted CEC, voltage assisted high performance microliquid chromatography (mu-HPLC), ON-CHIP and multidimensional electrochromatography. A number of detection methods, primarily mass spectrometry (in hybrid instruments with built-in microfluidic chips) are considered.