Institute for Analytical Instrumentation RAS, Saint-Petersburg
This paper is an extended version of the Russian
translation from English of the review article "Capillary Electrochromatography
of Peptides and Proteins" by Yan Li et al. ("Electrophoresis", 2004, 25,
2242-2256). This is an overview of recent advances in bioanalysis using
capillary electrochromatography (CEC) for separation of proteins and peptides.
Basic principles of CEC are briefly described. Much attention is given
to the development of new stationary phases and new column designs, their
advantages and disadvantages. New CEC column technologies, including monolithic
ones, recently developed to achieve high speed and efficiency of separation
are considered. Various analytical CEC platforms are introduced, namely:
pressure assisted CEC, voltage assisted high performance microliquid chromatography
(mu-HPLC), ON-CHIP and multidimensional electrochromatography. A number
of detection methods, primarily mass spectrometry (in hybrid instruments
with built-in microfluidic chips) are considered.