A. A. Evstrapov, G. E. Rudnitskaya, N. A. Petukhova*
Institute for Analytical Instrumentation RAS, Saint-Petersburg
*Institute of High-Molecular Compounds RAS, Saint-Petersburg
According to recent publications, one of the most promising
methods of DNA analysis is that of capillary electrophoresis on a microchip.
Many companies give much attention to the development of microchip tech-nologies
and instruments for rapid analysis of biological substances. The main trends
found in the development of microchip devices and systems include fabrication
of multichannel instruments, integration of auxiliary ele-ments on a microchip,
incorporation of microchip devices into analytical instruments (high-resolution
micro-scopes, mass spectrometers, etc.). One of the problems in methods development
for DNA analysis based on mi-crochip technology is the choice and synthesis
of the separation matrix for efficient fragmentation. The paper presents
the results of investigations on synthesis of poly(dimethyl acrylamide) with
prescribed properties. The data obtained from electrophoretic separation
of a mixture of DNA molecular weight markers on a glass micro-chip with different
characteristics of the separation medium are discussed.