A. A. Shcheglov, A. Yu. Shmykov, V. G. Maltsev
Institute for Analytical Instrumentation RAS, Saint-Petersburg
Chemical modification of the capillary
inner surface by means of grafting poly(sulfopropylmethacrylate) has been
suggested in order to stabilize the electroosmotic flow (EOF). It is shown
that such modified stable coatings provide independence of EOF rate from
pH as well as the absence of hysteresis typical for untreated silica capillaries.
By the example of serum protein analysis, it has been demonstrated
that poly(sulfopropyl-methacrylate) coating also provides increased resolution
of protein fractions and eliminates the necessity of capillary rinse with
alkali solution before and after analysis.