A. V. Novikov, N. V. Savel'eva*, N. V. Krasnov,
Peter Roepstorff**,
Roman Koerner**, O. A. Mirgorodskaya*
Institute for Analytical Instrumentation RAS, Saint-Petersburg
*Institute of Cytology RAS, Saint-Petersburg
**University of Southern Denmark, Denmark, Odense
A method of quantitative determination
of peptides and proteins by ESI-MS at picomolar levels was developed. The
essence of the method consists in determination of the concentration of
individual amino acids from the analyzed proteins. Proteins were first
subjected to hydrolysis (acid or enzymatic) and then we measured
relative ion intensities of amino acids and their isotope labeled analogs.
As internal standards, the Î18-containing analogs for 5 amino acids,
and also deuterated lysin and arginine modified by nitrogen 2N15 were used.
The method is applicable to proteins with known amino acid sequences. This
method was tested by the determination of concentration of bovine serum
albumin (BSA), chemotrypsinogen and proinsulin.