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"Nauchnoe Priborostroenie", 2020, Vol. 30, no. 4. ISSN 2312-2951, DOI: 10.18358/np-30-4-2076

"NP" 2020 year Vol. 30 no. 4.,   ABSTRACTS

ABSTRACTS, REFERENCES

A. G. Borodinov, V. V. Manoilov, I. V. Zarutsky, A. I. Petrov, V. E. Kurochkin

GENERATIONS OF DNA SEQUENCING METHODS (REVIEW)

"Nauchnoe priborostroenie", 2020, vol. 30, no. 4, pp. 3—20.
doi: 10.18358/np-30-4-i320
 

Several decades have passed since the development of the revolutionary DNA sequencing method by Frederick Sanger and his colleagues. After the Human Genome Project, the time interval between sequencing technologies began to shrink, while the volume of scientific knowledge continued to grow exponentially. Following Sanger sequencing, considered as the first generation, new generations of DNA sequencing were consistently introduced into practice. Advances in next generation sequencing (NGS) technologies have contributed significantly to this trend by reducing costs and generating massive sequencing data. To date, there are three generations of sequencing technologies. Second generation sequencing, which is currently the most commonly used NGS technology, consists of library preparation, amplification and sequencing steps, while in third generation sequencing, individual nucleic acids are sequenced directly to avoid bias and have higher throughput. The development of new generations of sequencing has made it possible to overcome the limitations of traditional DNA sequencing methods and has found application in a wide range of projects in molecular biology. On the other hand, with the development of next generation technologies, many technical problems arise that need to be deeply analyzed and solved. Each generation and sequencing platform, due to its methodological approach, has specific advantages and disadvantages that determine suitability for certain applications. Thus, the assessment of these characteristics, limitations and potential applications helps to shape the directions for further research on sequencing technologies.
 

Keywords: nucleic acid sequencing, genome research, generations of DNA sequencing technologies, directions of sequencing technologies research

Fig. 1. Maxam-Gilbert sequencing is based on specific cleavage of a DNA strand to produce labeled DNA fragments of various sizes [3]

Fig. 2. Sanger sequencing schematic diagram [7]

Fig. 3. Automatic DNA sequencing.
a – capillary electrophoresis system; á – laser detection of fluorescent labels; â–- electrophoretogram of DNA sequence [9]

Fig. 4. The diagram represents the functional principle of pyrosequencing

Fig. 5. Massive parallel pyrosequencing with the use of Roche 454 [12]

Fig. 6. SBS sequencing scheme [14]

Fig. 7. The principle of ABI / SOLID operation [20]

Fig. 8. The principle of Ion Torrent sequencing platform operation [21]

Fig. 9. The principle of Single Molecule Real Time (SMRT) Sequencing Technology operation [25]

Fig. 10. The principle of Single Molecule Real Time (SMRT) Sequencing Technology operation [28]

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Borodinov Andrey Gennadyevich, borodinov@gmail.com
Article received by the editorial office on 27.10.2020

Full text (In Russ.) >>

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  21. Golan D., Medvedev P. Using state machines to model the Ion Torrent sequencing process and to improve read error rates. Bioinformatics, 2013, vol. 29, no. 13, pp. i344—i351. DOI: 10.1093/bioinformatics/btt212
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N. A. Esikova, N. N. Germash, A. A. Evstrapov

RAPID FABRICATION OF MICROCHIPS FOR PCR ANALYSIS FROM POLYMER MATERIALS IN THE LABORATORY CONDITIONS

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 21—26.
doi: 10.18358/np-30-4-i2126
 

Polycarbonate and polypropylene microchips were made by thermoforming and sealed by special PCR films. The aim of this work was to study the possibility of promptly manufacturing microchips for performing RT-PCR analysis. Microchips are made of hard plastics, preferably using domestically produced materials.
Therefore, to achieve the goal, the following tasks were set: 1) thermoforming the topology of the chambers in polycarbonate and polypropylene master mold made by laser microforming; 2) sealing the obtained microchips with commercial films for PCR, which are polymer films with a thin adhesive layer (the connection should provide a binding force, holding the pressure arising in the chambers during thermal cycling, and not inhibiting the reaction); 3) study of light transmission and background fluorescence of microchips, since it is supposed to detect PCR-RT by optical methods; 4) study of the properties of the surface of the chambers of microchips (by the recumbent drop method), since they affect the formation of air bubbles during thermal cycling; 5) approbation of microchips when performing PCR-RT in them.
The efficiency of microchips was demonstrated by conducting PCR-RT on a test object. The obtaining microchips made of polycarbonate and polypropylene could withstand storage at negative temperatures (–40 ℃).
 

Keywords: microchip, thermoplastic, polycarbonate, polypropylene, polymerase chain reaction in real time, optical
detection

Fig.1. Image of the microchip topology with the numbering of the reaction chambers.
Dimensions are given in mm

Tab. 1. Estimation of the size of the obtained microchips

Fig. 2. Spectral dependences of the light transmission of microchips: averaged and with an average deviation (n = 6).
1 – ÏK + P-500; 2 – ÏK + Epp; 3 – ÏÏ + P-500

Tab. 2. Intensity of background fluorescence of microchips (in rel. units)

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Esikova Nadezhda Aleksandrovna, elpis-san@yandex.ru
Article received by the editorial office on 26.10.2020

Full text (In Russ.) >>

REFERENCES

  1. Yin Z., Zou H., Sun L. Polycarbonate nanofluidic chip fabrication technique by hot embossing and thermal bonding. J. Nanosci. Nanotechnol,. 2018, vol. 18, no. 4, pp. 2530—2535. DOI: 10.1166/jnn.2018.14341
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D. O. Kuleshov1, I. A. Gromov2, E. N. Alekseyuk3, A. V. Solov'eva3, N. R. Gall2, L. N. Gall1

INVESTIGATION OF THE POSSIBILITY OF USING THE LIQUID SURFACE AS AN ELECTRODE FOR COLLECTING PRODUCTS OF CHEMICAL REACTIONS OCCURRING IN THE ELECTROSPRAY TORCH MICRODROPLETS

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 27—31.
doi: 10.18358/np-30-4-i2731
 

The present work is devoted to the study of the possibility of electrospray onto the surface of a liquid in order to accumulate the products of reactions occurring in micro- and nanodroplets of the electrospray torch. The experimental device was made for research purposes. A stable mode of electrospray of a water-ethanol mixture (1:4) onto a liquid electrode (water-ethanol mixture (1:4)), without using sputting gas, was obtained at a spray voltage U of at least 4 kV and a distance between the spray needle and the surface of the liquid R of no more than 15 mm. The spray current increased as the distance between the spray needle and the surface of the liquid electrode decreased and the spray voltage increased. A stable mode of electrospray of the reaction mixture (4 ml of a mixture of acetonitrile and 0.2 % formic acid solution in water (1: 1), 0.5 ml of aniline and 0.5 ml of acetone) onto the surface of the liquid electrode (mixture of 6 ml of acetonitrile and 6 ml of 0.2 % formic acid solution in water or a mixture of 6 ml of acetonitrile and 6 ml of water) with the use of the sputting gas was obtained with the following values of the parameters of the experimental device: the flow rate of the spray solution Qp = 100 μL / min, the spray voltage U = 3.2 kV, the distance between the surface of the liquid electrode and the spray needle R = 35 mm. The maximum flow rate of the sputting gas was 2 L/min. Unreacted substances and the chemical reactions products of the components of the sprayed reaction mixture accumulated in the liquid electrode when the given values of the parameters of the device were used. This was confirmed by spectrophotometric measurements.
 

Keywords: electrospray, electrospray torch, microdroplets, chemical reactions, liquid electrode

Fig. Diagram of the experimental setup.
1 – syringe pump, 2 – spray capillary, 3 – inner needle (outer diameter 0.45 mm), 4 – outer needle (outer diameter 0.8 mm), 5 – compressor, 6 – pressure hose, 7 – metal holder, 8 – high-voltage power supply, 9 – ring electrode, 10 – storage of reaction products, 11 – digital multimeter No. 1; 12 – digital multimeter No. 2; 13, 14 – potential of the Earth

Tab. Dependence of the spray current value on the flow rate of the sputting gas.
Qair – flow rate of the sputting gas, l/min; IP —spray current, nA

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Kuleshov Denis Olegovich, hellchemist@yandex.ru
Article received by the editorial office on 4.11.2020

Full text (In Russ.) >>

REFERENCES

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V. E. Kurochkin, B. P. Sharfarets

GIANT DISPERSION OF DIELECTRIC PERMEABILITY OF THE DISPERSE SYSTEM IN AN ALTERNATING ELECTRIC FIELD.
OVERVIEW OF APPROACHES TAKING INTO ACCOUNT PRESENCE OF A DOUBLE LAYER

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 32—45.
doi: 10.18358/np-30-4-i 3245
 

This review analyzes two approaches to describing the polarization of disperse systems when an alternating electric field is applied in the general case. Polarization models of Trukhan and Dukhin–Shilov are considered. As a result of this review, it follows that the most preferable model, which makes it possible to significantly increase the rate of electrophoresis of the dispersed phase, is the Dukhin–Shilov model, in the implementation of which a giant dispersion of the dielectric constant of a heterogeneous dispersed system may appear if the condition of a thin double layer around non-conducting dispersed particles in a conducting dispersive medium.
 

Keywords: the dielectric constant, giant dispersion of the dielectric constant, electrophoresis, thin double layer approximation, heterogeneous environment, Maxwell–Wagner polarization, dispersion system

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Sharfarets Boris Pinkusovich, sharb@mail.ru
Article received by the editorial office on 28.08.2020

Full text (In Eng.) >>

REFERENCES

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B. P. Sharfarets

ELECTROPHORESIS IN THE TOTAL (CONSTANT AND ALTERNATING) ELECTRIC FIELD.
II. PECULIARITIES OF THE COMBINED IMPACT OF ALTERNATING AND CONSTANT ELECTRIC FIELDS

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 46—51.
doi: 10.18358/np-30-4-i4651
 

The hydrodynamics of electrophoresis under the simultaneous impact of constant and alternating electric fields is considered. It has been shown that when the constant and alternating external fields are combined, the energy of the constant electric field is transferred into the alternating hydrodynamic field. An example is given of a dispersed medium in which a giant dispersion of the dielectric constant can arise, which in turn can contribute to an increase in the total electrophoresis rate. Analogies of the behavior of the considered dispersed medium with the action of an electroacoustic transducer based on the use of electrokinetic phenomena are given.
 

Keywords: electrophoresis, electric field, dispersed particle, dispersed environment, variable flows, stationary flows, the Navier—Stokes equation

Author affiliations:

Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Sharfarets Boris Pinkusovich, sharb@mail.ru
Article received by the editorial office on 22.09.2020

Full text (In Eng.) >>

REFERENCES

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  6. Sergeev V.A., Sharfarets B.P. [About one new method of electroacoustic transformation. A theory based on electrokinetic phenomena. Part II. The acoustic aspect]. Nauchnoe Priborostroenie [Scientific Instrumentation], 2018, vol. 28, no. 2, pp. 36—44. DOI: 10.18358/np-28-2-i3644 (In Russ.).
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  10. Sharfarets B.P., Kurochkin V.E. [Giant dispersion of dielectric permeability of the disperse system in an alternating electric field. Overview of approaches taking into account presence of a double layer]. Nauchnoe Priborostroenie [Scientific Instrumentation], 2020. Vol. 30, no. 4. (In Russ.).
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  14. Sharfarets B.P. [Application of the system of electrohydrodynamics equations for mathematical modeling of a new method of electro-acoustic transformation]. Nauchnoe Priborostroenie [Scientific Instrumentation], 2018, vol. 28, no. 4, pp. 127—134. DOI: 10.18358/np-28-4-i127134 (In Russ.).
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  16. Sharfarets B.P., Kurochkin V.E., Sergeev V.A., Dmitriev S.P., Telyatnik S.G. [About electroacoustic transducer based on the use of electrokinetic phenomena]. Trudy vserossiyskoy akusticheskoy konferencii [Proceedings of the All-Russian Acoustic Conference]. Saint-Petersburg, Politekhpress, 2020, pp. 445—450. (In Russ.).
 

M. V. Duykova1, S. E. Shkonda1, S. A. Kazakov2, M. A. Grevtsev2

MANUFACTURING AND RESEARCH OF METAL OXIDE
SEMICONDUCTOR GAS SENSORS FOR AMMONIA

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 52—62.
doi: 10.18358/np-30-4-i5262
 

Ammonia-sensitive materials based on tin dioxide have been developed and synthesized. The degree of structural defects was evaluated, and the acid-base surface centers of synthesized materials were studied using the indicator method. The relationship between the chemisorption properties of synthesized gas-sensitive sensor layers (gas response to the concentration effect of ammonia) and the structure of the sensor material is discovered and analyzed. The novelty of the research is the integrated approach presented in this paper to the creation, development and manufacture of sensors for ammonia by synthesizing a material with pre-set properties using several methods of surface modernization simultaneously.
 

Keywords: gas analyzer, semiconductor sensor, ammonia detection, chemisorption, defective structure, x-ray diffraction analysis

Author affiliations:

1Scientific and Production Association PRIBOR, JSC, Saint Petersburg, RF
2Ioffe Physical Technical Institute of the RAS, Saint Petersburg, RF

 
Contacts: Duikova Margarita Vadimovna, mduykova@bk.ru
Article received by the editorial office on 18.09.2020

Full text (In Eng.) >>

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N. A. Gryaznov, D. A. Goryachkin, V. I. Kuprenyuk, E. N. Sosnov, V. L. Alekseev

PASSIVE STABILISATION OF MICHELSON INTERFEROMETER

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 63—74.
doi: 10.18358/np-30-4-i6374
 

In some applications of a Michelson interferometer, in particular, when it is used in a laser resonator, the phase difference of radiation beams passing the interferometer optical branches must be stabilized with high accuracy. The proposed paper is devoted to experimental studying the long-term stability of several interferometer designs and choosing the optimal version for applying as a compound resonator mirror with the controllable reflection for generation of ultrashort laser pulses.
 

Keywords: Michelson interferometer (MI), the optical paths difference (OPD), stabilization, random and systematic intensity fluctuations

Author affiliations:

The Russian State Scientific Center for Robotics and Technical Cybernetics (RTC), Saint Petersburg, RF

 
Contacts: Goryachkin Dmitry Alekseevich, d.goryachkin@rtc.ru
Article received by the editorial office on 15.10.2020

Full text (In Eng.) >>

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P. A. Kupriy1, M. Z. Muradymov2, N. V. Krasnov2, I. V. Kurnin2, A. N. Arseniev2

EFFECT OF GAS-DYNAMIC FLOW ON ION TRANSPORT THROUGH THE NOZZLE
OF AN ION SOURCE WITH IONIZATION AT ATMOSPHERIC PRESSURE

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 75—83.
doi: 10.18358/np-30-4-i7583
 

The effect of the gas flow to the ion transport from the atmosphere to the forevacuum region of the is determined experimentally. Experimental volt-ampere characteristics of the ion flow carried by the gas-dynamic flow behind the nozzle are obtained. It is shown that the fraction of the ion current passing through the nozzle under the influence of the gas flow can be increased several times in relation to the current value due only to the extraction by the electric field.
In this case, depending on the magnitude of the electric field penetrating beyond the skimmer, the current is redistributed between the nozzle and the collector. It was found that, under the experimental conditions, the entire ion flux reaches the movable collector at a distance of up to 15 mm at a field strength of 200 V / cm. The diameter of the ionic component of the flow is 1.5 mm.
The ion motion in a gas is simulated for conditions close to the experimental ones. Developing research findings, it is possible to consider systems for transporting ions further to the analyzer.
 

Keywords: ion source, gas dynamic flow, nozzle, ion transport at high gas pressure

Fig. 1. Graph of losses (in %) of ions during transportation from the source to the analyzer [2]

Fig: 2. Diagram of the experimental setup.
d1—d4 – diaphragms; 5 – fluoroplastic gaskets; 6 – vacuum chamber; 7 – Wilsonian seal; 8 – collector (rod); 9 – emitter (needle); 10 – forvacuum pump; 11 – vacuum pressure sensor; 12 – valve; 13, 14 – highly stable power supplies; 15—20 – electrometers; 21—26 – digital current meters

Fig: 3. Dependence of currents on diaphragms I1, I2, I3, I4, collector Iê and their sum on the collector Uê
voltage at a distance from the needle to the first diaphragm of 5 mm and discharge current 1 μA

Fig. 4. Dependence of the currents on the fourth diaphragm I4 and the collector Iê on the collector Uê voltage at a distance from the needle to the first diaphragm of 5 mm and a discharge current of 1 μA

Fig. 5. Dependences of the currents at the nozzle (fourth diaphragm) I4 and the collector Iê on the collector Uê voltage at a discharge current of 1 μA and the distance from the nozzle to the collector (a) 5 mm, (á) 10 mm, (â) 15 mm

Fig. 6. Dependences of the currents at the nozzle (fourth diaphragm) I4 and the collector Iê on the voltage on the collector Uê at a discharge current of 2 μA and a distance from the nozzle to the collector (a) 5 mm, (á) 10 mm

Fig. 7. Characteristic trajectories of ions in the area of the outlet diaphragm.
a – at atmospheric pressure and a collector potential of —300 V, b – at collector zero potential, taking into account the gas flow rate (air pressure behind the diaphragm is 8 Torr)

Fig. 8. Spatial distribution of the gas flow velocity in the ion transport system at a pressure in the chamber on the right (a) 8 Torr and (b) 2 Torr. Diaphragm hole diameter is 0.5 mm

Fig. 9. Normalized dependences of the collector current value on its potential. Designated: transportation system at atmospheric pressure (Atm), when pumping out the area behind the last diaphragm to a pressure of 8 Torr (dP)

Author affiliations:

1Peter the Great Saint-Petersburg Polytechnic University, Saint Petersburg, Russia
2Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia

 
Contacts: Krasnov Nikolay Vasil'evich, krasnov@alpha-ms.com
Article received by the editorial office on 28.10.2020

Full text (In Russ.) >>

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A. G. Kuzmin1, Yu. A. Titov1, N. B. Suvorov2, M. V. Kuropatenko2

MASS-SPECTROMETRIC STUDIES OF THE DYNAMICS OF EXHALED AIR COMPOSITION DURING DYNAMIC POSTURAL EFFECTS

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 84—93.
doi: 10.18358/np-30-4-i8493
 

The paper studies the possibility of using a quadrupole gas mass spectrometer as part of a diagnostic complex based on a rotary (mechanorgical) table to assess the effectiveness of the dynamic postural effects on patients. Analysis of the obtained mass spectrograms indicates that the response of patients is individual and can vary significantly. Taking into account the mass spectrometric parameters of exhaled air allows the patient to be assigned to a specific group. Information on the concentration of oxygen and carbon dioxide in the patient's exhalation, as well as the analysis of the envelope shape of the measured dynamic mass spectrograms can be used for comprehensive diagnostics of the patient's condition in addition to the data of the main sensors in the complex.
 

Keywords: mass spectrometry, exhaled air analysis, postural influences, mechanorgic table

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint-Petersburg, Russia
2Federal State Budgetary Scientific Institution "Institute of experimental medicine",
Saint-Petersburg, Russia

 
Contacts: Kuzmin Aleksey Georgievich, agqz55@rambler.ru
Article received by the editorial office on 09.10.2020

Full text (In Eng.) >>

REFERENCES

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  6. Kuzmin A.G., Tkachenko E.I., Oreshko L.S., Titov Yu.A., Balabanov A.S. [Mass spectrum method of metrical express diagnostics by exhaled air composition]. Medicinskij akademicheskij zhurnal [Medical Academic Journal], 2016, vol. 16, no. 4, pp. 106—112. URL: https://iemspb.ru/wp-content/uploads/2016/12/MAZH_No_4-2016_full.pdf (In Russ.).
 

L. V. Novikov1, V. V Manoilov1, A. G. Kuzmin1, Yu. A. Titov1, I. V. Zarutsky1, A. O. Nefedov2,
A. V. Nefedova2, A. I. Arseniev3

EXPRESS DIAGNOSTICS OF DISEASES BASED ON A QUADRUPOLE MASS SPECTROMETER ANALYSIS OF EXHALED AIR

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 94—105.
doi: 10.18358/np-30-4-i94105
 

The method is proposed for express diagnostics of diseases according to the data of mass spectrometric analysis of The method is proposed for express diagnostics of diseases according to the data of mass spectrometric analysis of exhaled air. An algorithm for calculating the probability of diseases has been developed and tested. The results of data processing of patients treated in two oncological clinics are presented. The calculation of the probability of disease according to the data of mass spectrometric analysis of exhaled air is based on attributing the mass spectrum of the tested patient to the mass-spectra of the corresponding control group. Each control group is formed by collecting an array of spectra from at least ten patients with the same disease. Diagnostics is performed by transforming the matrix of spectra of the control group and the spectrum of a patient being tested into the space of the principal components. The probability of a disease is determined by the Euclidean distance of the patient's coordinates from the centroid of the control group in the multidimensional space of these principal components.
 

Keywords: express diagnostics of the disease, principal component analysis, multivariate probability density, multivariate data processing

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia
2St. Petersburg Clinical Scientific and Practical Center for Specialized Types
of Medical Care (Oncological), Russia
3Scientific Research Center of Oncology named after N.N. Petrov Ministry of Health of Russia

 
Contacts: Manoilov Vladimir Vladimirovich, manoilov_vv@mail.ru
Article received by the editorial office on 15.10.2020

Full text (In Eng.) >>

REFERENCES

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A. Yu. Zaitceva1, M. S. Mazing1,2, Yu. Ya. Kislyakov1

MULTISENSOR OPTICAL SYSTEM FOR NON-INVASIVE MONITORING OF THE HUMAN OXYGEN BODY DURING FUNCTIONAL STRESS

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 106—112.
doi: 10.18358/np-30-4-i106112
 

This work was performed using optical spectroscopy methods. The proposed method is based on the differences in the absorption spectra of oxyhemoglobin and reduced hemoglobin, as well as on the analysis of the response of the system using mathematical methods for processing multidimensional data. In the course of the work, a prototype of the diagnostic optical system for non-invasive monitoring of the oxygen supply of human tissues was created, which is based on a multi-channel optical spectrum analyzer. As a result of experimental studies, it was shown that each subject has his own individual, reproducible "image". The results of the study confirm the effectiveness of the proposed method for assessing the functional state of a person and the prospects for its use in practical medicine.
 

Keywords: spectrophotometry, non-invasive diagnostic method, oxygen tissue supply, functional state, optical system

Fig. 1. External view of the analyzer of optical spectra with off (a) and on (á) LEDs

Fig: 2. Oxygenation curve with marked time points of measurements during the experiment.The time of measurement was noted as t2 – holding the breath, t3 – resuming breathing, t4 – 60 s after the delay (restart), t5 – 240 s after the delay (restart) [5]

Tab. 1. Obtained averaged measurements of the device with standard deviation at rest, recorded for 15 s in five subjects

Tab. 2. Obtained averaged measurements of the device with SD during the recovery period, in 240 s after the functional stress in five subjects

Fig. 3. Visual representation of subjects in the form of hexagons. The axes of the diagram show the numerical readings of each of the six sensors in relative units

Fig. 4. Visual representation of the average normalized image of the subjects of the first group. The axes of the diagram show the standardized numerical readings of each of the six sensors

Fig. 5. Visual representation of the average normalized image of the subjects of the second group. The axes of the diagram show the standardized numerical readings of each of the six sensors

Fig. 6. Visual representation of the averaged normalized image of the subjects of the third group. The axes of the diagram show the standardized numerical readings of each of the six sensors

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia
2Peter the Great Saint Petersburg Polytechnic University, Saint Petersburg, Russia

 
Contacts: Zaitceva Anna Yur'evna, anna@da-24.ru
Article received by the editorial office on 30.10.2020

Full text (In Russ.) >>

REFERENCES

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A Yu Zaitceva1, Yu. Ya. Kislyakov1, M. S. Mazing1,2, V. V. Davydov2, 3

APPLICATION OF NON-INVASIVE OPTICAL TRAINABLE DIAGNOSTIC SYSTEM AND MATHEMATICAL METHODS OF PROCESSING MULTIDIMENSIONAL DATA TO ASSESS THE OXYGEN STATUS OF HUMAN TISSUES (SHORT DESCRIPTION)

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 113—118.
doi: 10.18358/np-30-4-i113118
 

A non-invasive method for analyzing oxygen supply for human tissues using methods of mathematical processing of multidimensional data, which has an important diagnostic value, is proposed. As a result of experimental studies with the participation of 10 subjects using a non-invasive optical trainable diagnostic system, attempts were made to collect information in conventional units by means of multichannel optical analyzer of the visible spectral range. The obtained results of mathematical processing of experimental data show that the proposed technique is effective and can be used in practical medical and biological
 

Keywords: optical system, multisensor systems, functional state assessment, non-invasive diagnostic method

Fig. 1. Functional block diagram of a non-invasive optical trainable diagnostic system

Fig. 2. Average normalized images of the oxygen status of tissues of two groups of subjects (a, á)

Fig. 3. The result of clustering the oxygen images of 10 subjects in the space of two principal components by the method of k-means. The images were formed by the method of principal components according to 6 features, which are the numerical readings of each of the six sensors after functional load, normalized in regard to the values obtained at rest. The first principal component is along the x-axis is and the second principal component is along the y-axis

Author affiliations:

1Institute for Analytical Instrumentation of RAS, Saint Petersburg, Russia
2Peter the Great Saint Petersburg Polytechnic University, Russia
3Department of Ecology, Russian Research Institute for Phytopathology, Moscow Region, Odintsovo

 
Contacts: Zaitceva Anna Yur'evna, anna@da-24.ru
Article received by the editorial office on 16.10.2020

Full text (In Russ.) >>

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  2. Kislyakov Yu.Ya., Avdyushenko S.A., Kislyakova L.P., Zaitceva A.Yu. Analytical multisensory trainable system for diagnosing vocational aptitude of military medical specialists by ion content in the expired breath condensate. Journal of Computational and Theoretical Nanoscience, 2019, vol. 16, no. 11, pp. 4502—4507. DOI: 10.1166/jctn.2019.8343
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CONTENTS OF VOLUME 30

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 119–126.
doi: 10.18358/np-30-4-i119126
 
 NUMBER 1 (80 p.)
 PHYSICS OF INSTRUMENT MAKING (pp. 3–38)
 SYSTEM ANALYSIS OF MEASURING DEVICES AND METHODS (pp. 39–67)
 MATHEMATICAL METHODS AND MODELLING IN INSTRUMENT MAKING (pp. 68–79)
 FROM EDITION (pp. 80–80)
 
 NUMBER 2 (76 p.)
 INSTRUMENT MAKING OF PHYSICAL AND CHEMICAL BIOLOGY (pp. 3–32)
 PHYSICS OF INSTRUMENT MAKING (pp. 33–50)
 MATHEMATICAL METHODS AND MODELLING IN INSTRUMENT MAKING (pp. 51–60)
 INFORMATICS, COMPUTER TECHNICS AND CONTROL (pp. 61–75)
 
 NUMBER 3 (76 p.)
 PHYSICS OF INSTRUMENT MAKING (pp. 3–18)
 INSTRUMENT MAKING OF PHYSICAL AND CHEMICAL BIOLOGY (pp. 19–48)
 INFORMATICS, COMPUTER TECHNICS AND CONTROL (pp. 49–76)
 
 NUMBER 4 (128 p.)
 INSTRUMENT MAKING OF PHYSICAL AND CHEMICAL BIOLOGY (pp. 3–38)
 PHYSICS OF INSTRUMENT MAKING (pp. 39–88)
 INSTRUMENT MAKING FOR BIOLOGY AND MEDICINE (pp. 89–118)
 
 Contents of Volume 30     (pp. 119–126)
 Authors Index of Volume 30 (pp. 127–128)

Full text (In Eng.) >>
 

AUTHORS INDEX OF VOLUME 30

"Nauchnoe Priborostroenie", 2020, vol. 30, no. 4, pp. 127—128
doi: 10.18358/np-30-4-i127128

Abiev R. Sh. — ¹ 1
Aleksandrova M. L. — ¹ 2
Alekseev V. L. — ¹ 4
Alekseyuk E.N. — ¹ 4
Antonov R.Yu. — ¹ 3
Anufriev A. V. — ¹ 1
Archipov D. B. — ¹ 3
Archipov N.D. — ¹ 3
Arsen'ev A. I. — ¹ 4
Arsen'ev A. N. — ¹ 4
Batozyrenova E. G. — ¹ 1
Belov D. A. — ¹ 2
Belov Yu. V. — ¹ 2
Belyaeva O. A. — ¹ 1
Berdnikov A. S. — ¹ 2
Borodinov A.G. — ¹ 4
Bublyaev R. — ¹ 1
Bulyaniza A. L. — ¹ 3
Buryak A.K. — ¹ 3
Chabarov V.B. — ¹ 3
Chernyakov I.S. — ¹ 3
Chudyakov A. V. — ¹ 1
Esikova N. A. — ¹ 4
Evstrapov A. A. — ¹ 3, 4
Fofanov Ya. A. — ¹ 1
Gaft S.S. — ¹ 2
Gall I.R. — ¹ 2
Gall L. N. — ¹ 2, 4
Gall' N. R. — ¹ 1, 2, 4
Gavrilov D. A. — ¹ 2
Germash N. N. — ¹ 4
Gladchuk A. S. — ¹ 1
Gladchuk A. S. — ¹ 1, 4

Gorbacheva T.T. — ¹ 2
Gorbacheva T.T. — ¹ 4
Goryachkin D. A. — ¹ 4
Goryachkin D. A. — ¹ 4
Grevzev M. A. — ¹ 4
Grevzev M. A. — ¹ 4
Kazakov S. A. — ¹ 4
Kel'zieva O. A. — ¹ 2
Kislyakov Yu. Ya. — ¹ 4
Krasheninnikov V. N. — ¹ 1
Krasnov N. V. — ¹ 4
Kuleshov D. O. — ¹ 1, 4
Kuprenyuk V. I. — ¹ 4
Kupriy P. A. — ¹ 4
Kurnin I. V. — ¹ 1, 4
Kurochkin V. E. — ¹ 1,2,3,4
Kuropatenko M. V. — ¹ 4
Kuz'min A. G. — ¹ 4
Kuz'min Yu. I. — ¹ 1
Lisin D. V. — ¹ 2
Maksimov S. I. — ¹ 2
Manoylov V. V. — ¹ 4
Mayorov D.V. — ¹ 2
Mazing M. S. — ¹ 4
Mirgorodskaya O. A. — ¹ 1
Muradymov M. Z. — ¹ 4
Nazarov D.I. — ¹ 3
Nefedov A. O. — ¹ 4
Nefedova A. V. — ¹ 4
Novikov D.V. — ¹ 1
Novikov L. V. — ¹ 4
Petrov A. I. — ¹ 4
Pleshakov I. V. — ¹ 1

Podol'skaya E. P. — ¹ 1, 2
Popov Yu. A. — ¹ 2
Prokof'eva Yu. P. — ¹ 1
Protasov A. V. — ¹ 1
Prozorov A. A. — ¹ 2
Prozorova I. V. — ¹ 2
Reynyuk V.L. — ¹ 2
Schelkunov N.N. — ¹ 2
Scherbakov A. P. — ¹ 1
Sharfarez B. P. — ¹ 1,2,3,4
Sharipov S.U. — ¹ 2
Shkonda S. E. — ¹ 4
Shreyner E. V. — ¹ 2
Shugaeva T. Zh. — ¹ 2
Sil'kis E. G. — ¹ 1
Sokolov B.V. — ¹ 3
Solov'eva A. V. — ¹ 4
Sosnov E. N. — ¹ 4
Spivak-Lavrov I. F. — ¹ 2
Stankevich A. S. — ¹ 1
Suchodolov N. G. — ¹ 2
Suvorov N. B. — ¹ 4
Svetlov S. D. — ¹ 1
Titov Yu. A. — ¹ 4
Velyaev Yu.O. — ¹ 2
Zacharov V.V. — ¹ 3
Zaruzkiy I. V. — ¹ 4
Zayzeva A. Yu. — ¹ 4
Zhernovoy A. I. — ¹ 1
Zhukov A. N. — ¹ 1
Zubik A.N. — ¹ 2

Full text (In Eng.) >>

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