This article presents the results of using in clinical practice the analyser of thiol antioxidants to determine the state of thiol-disulfide system and the level of nonspecific resistance of organism in critical conditions and pregnancy. For the first time the quantitative values of thiol-disulfide coefficient depending on the form and disease severity as well as in the case of the complicated course pregnancy were reliably determined. It is shown that the state of thiol compounds activity allows to evaluate the efficiency of preoperative preparation of patients, to determine in the first postoperative days the possibility of complications development as well as to predict the course of pregnancy and conduct the preserving therapy during the treat of pregnancy termination. In this article the high demand and prospects of using instrumental means and methodological approaches for determining of the thiol-disulfide status of the organism, both in medical practice and in preventive medicine is shown.

¹Kirov Military Medical Academy, Saint-Petersburg
²North-West State Medical University named after I.I. Mechnikov, Saint-Petersburg
³Saint-Petersburg State Pediatric Medical University
⁴Maternity Hospital № 16, Saint-Petersburg
⁵Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: S. N. Gaydukov, gaiducovsn@rambler.ru

The present article describes the optimization procedure of metal-affinity chromatography on sorbents, containing ions of Ti (IV) (Supel-Tips Ti) for separation of peptides from the digest of human serum albumin, incubated with sarin. It is shown that changing the conditions recommended by the manufacturer, namely sorption of alkaline conditions rather instead of acidic, and elution of 0.1 % TFA in 60 % acetonitrile instead of 0.5 % pipe-ridine results in a more efficient isolation of phosphonylation peptides from the samples

¹Research Institute of Hygiene, Occupational Pathology and Human Ecology, Saint-Petersburg
²Institute for Analytical Instrumentation of RAS, Saint-Petersburg
³Institute of Toxicology, Saint-Petersburg
Contact: Ya. A. Dubrovskiy, dubrovskiy.ya@gmail.com

The present work develops our results obtained in recent studies [1–3] employing stochastic imitation modeling to analyze real-time PCR kinetics and DNA quantitative assay performance. In particular, PCR threshold cycle probability distribution depending on initial DNA copy number was estimated, reaction kinetics was studied, quantitative assay errors were analyzed. In the present paper, a number of topics related to the quantitative assay results interpretation is discussed: estimation of copy number (n) in the sample based on a series of quantitative assay results (k_i, i = 1, 2,…, m) for a number of assay runs; obtaining estimates k_i; interpretation of negative or false-negative assay results in the case of multiple (m) runs with no copies found.

¹Institute for Analytical Instrumentation of RAS, Saint-Petersburg
²Joint Stock Company "Syntol", Saint-Petersburg
Contact: D. G. Sochivko, sochivko@yahoo.com

It’s proposed to use an approximation of the tabulated dependence of the peaks position of the internal standard on the time by a polynomial of the fourth degree as an analytic function. This analytic function permits to plot a calibration line to determine the optimal length of the analyzed DNA fragments within the calibration range and beyond its range.

Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: Yu. V. Belov, bel3838@mail.ru

A technique for checking of the regular monitor inhalational anesthetic agent for the inhalational anesthesia apparatus is described. The verification was performed by means of a refractometer and a mass spectrometer in real time. The possibility of the use of small-sized refractometer for testing of the regular monitor for the inhalational anesthetic agent under the conditions of a clinic.

¹ZAO Nauchnye Pribory, Saint-Petersburg
²Ioffe Physical Technical Institute RAS, Saint-Petersburg
³Kirov Military Medical Academy, Saint-Petersburg
Contact: A. Yu. Elizarov, a.elizarov@mail.ioffe.ru

The paper describes two approaches for selecting of the optimum channel widths by a discretization of the velocity scale in the process of Mössbauer spectra accumulation by means of multichannel accumulating mechanisms. The first approach is based on an analysis of the Kotel’nikov—Shannon’s frequency criterion, the second approach is based on an analysis of errors that arise in the case of a differential representation of the spectrum. In this case, the analysis is conducted in two stages: in the case of the first step the shape distortion of the ideal spectrum due to discretization of the velocity scale is considered, but in the case of the second step the influence of the statistical scatter in the spectrum by introducing of the "spectrum quality" parameter (signal/noise relation) are taken into account. The mathematical expressions are derived for estimation the width of the channel for the each approach.

¹Institute for Analytical Instrumentation of RAS, Saint-Petersburg
²Saint-Petersburg State University
Contact: S. M. Irkaev, sobir_irkaev@mail.ru

The detailed analysis of force balance when insonifying the ultrasonic suspension water-filled column with sorbent micro particles inside it is performed. The analysis is carried out taking into account the presence of different velocities flowing streams in a camera. The conducted analysis permits more effective qualitative planning of experiments on retention and washing out micro particles of different nature.

¹V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry RAS, Moscow
²Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: B. P. Sharfarets, sharb@mail.ru

Two modes of cylindrical mirror type energy analyzer work: the minimum trace width mode and the mode in which the optimal aperture is the maximum one are investigated. It is shown that the last mode is one of the realizations of the minimum trace width mode. It is shown that with the right choice of the gap the output diaphragm, apparatus function in the minimum trace width mode has only one peak.

Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: S. I. Shevchenko, shevsi@yandex.ru

А method for calculating of the non-uniform electrostatic field of the mass reflection reflector is described. The method allows to calculate the axial potential distribution in the reflector theoretically eliminating dispersion of the total time of flight for axial achromatic ion packets in a given range of the initial energy of the ions. By the method of the numerical simulation the analysis of the errors influence for the field physical synthesis close to the real reflector on the focusing accuracy is carried out. The calculation procedure of the reflector with an inhomogeneous field is proposed. The description of the potentials optimization algorithm for field-determination elements is given. The recommendations to reduce execution time of the algorithm are given. The design used for verification of the optimization algorithm is described; the results of simulation of the focusing procedures for ions of different masses and different initial energy spreads are given.

Samara State Aerospace University named after academician S.P. Korolyov (National Research University)
Contact: D. V. Rodin, rodin.ssau@gmail.com

Jamin interferometer is chosen for functioning of processing system of holographic interferograms using dual-frequency radiation. The optical scheme of the light rays passing through the plates of Jamin interferometer and the scheme to the calculation of path difference of the rays in the interferometer are given. The results of the interferometer analysis in the strict narrative are obtained. The analytical expression for the phase difference of the rays passed through Jamin interferometer is derived.

¹Northwest State Medical University named after I.I. Mechnikov, Saint-Petersburg
²Saint Petersburg National Research University of Information Technologies, Mechanics and Optics
Contact: E. E. Maiorov, majorov_ee@mail.ru

On the basis of a cluster lattice model, the cross-sections of monolithic sorbents in fused-silica microcolumns for capillary electrochromatography were studied. The columns were obtained by polymerization of methacrylate monomers in solution under the influence of accelerated electrons. It was shown that the size distribution and packing density of microglobules on the surface cross-sections depend on the composition of a mixture of monomers and the content of porogenic solvents. At the threshold degree of surface coverage by globules, there is a fractal cluster function, for which the radial density distribution g (R) decreases with increasing radius R as a power law. An infinite cluster of pores, with a finite probability of the injected fluid flowing through the inter-connected free knots or percolated pores of the monolithic sorbent, is formed near the threshing surface coverage of a cross-section by microglobules.

¹Institute for Analytical Instrumentation of RAS, Saint-Petersburg
²Institute of Macromolecular Compounds of the Russian Academy of Sciences, Saint-Petersburg
³Saint-Petersburg State Technological Institute
⁴Technology Center RADIANT, Saint-Petersburg
⁵Saint-Petersburg National Research University of Information Technologies, Mechanics and Optics,
⁶Saint-Petersburg State University of Engineering and Economics
Contact: A. N. Krasovskii, alex-krasovski@yandex.ru

Theoretical and experimental investigations of magnetic field penetration in the granular superconductors of YBa₂Cu₃O_7-x composition are presented. On the basis of the investigations results the assessment of a noise magnetic stream is carried out, the assumption of limiting sensitivity of magnetic field sensors on the ground of high-temperature superconductors is made too.

Volgograd State University
Contact: S. V. Chernykh, schtks@list.ru

In experiment, the effective coefficient of demagnetization the sample of colloidal solution ferromagnetic nanoparticles was measured by method of NMR. This solution was put in two long cylindrical tubes parallel to intensity of external magnetic field and having diameters are 10 times less than the height of the liquid. It is found that the measured coefficient of demagnetization N ≈ 0.09, while in theory N = 0.02. The effect is explained by the presence of conglomerates of nanoparticles.

Saint-Petersburg State Technological Institute (Technical University)
Contact: A. I. Zhernovoy, azhspb@rambler.ru

The article presents the results of the researches, dedicated to the temperature dependence of the pressure electrical resistivity coefficient (PRC) of the baroresistor on the ground of thin film of the samarium monosulphide (SmS) on the glass substrate (SiO₂) within the range of 77–350 K. The feature as a sharp maximum is observed throughout the temperature dependence of the PRC module. This maximum can be explained by the growing activity of electrons conductivity from the donor level, which energy depends on the temperature deformation, appearing as the result of the temperature coefficients difference between linear dilatation of the film and glass substrate. The calculation of the activation energy according to this donor level is carried out. On the basic of the work results the acceptable for the technical using the low temperature border for tenso- and baroresistor operation on the ground of the SmS films equal to the ~150 К is determined.

¹Ioffe Physicotechnical Institute RAS, Saint-Petersburg
²Federal Enterprise "Lavochkin Association", Moscow region, Khimki
Contact: N. N. Stepanov, stnick@hotbox.ru

The method for the construction of devices for generating ultra wideband pulse and harmonic signals with parameters that can be regulated with high precision.

Omsk State Technical University
Contact: A. V. Nikonov, nalva@mail.ru

A hardware-oriented algorithm of median filtering and structures to realize it are presented. The solution enables ultrafast definition of data median inside oserved window due to parallel execution of comparisons and ordering. The approach provides a cost-effective implementation, significantly accelerates the median filtering and expands the scope of the method.

Saint-Petersburg Electrotechnical University "LETI"
Contact: A. Kh. Mursaev, sashamursaev@yandex.ru

In TEU with heat in the form of radiation, rather than the Peltier heat, the energy of the hot junctions converted into photons. This allows you to change the length of the branches and make TEU as a thin film structure. As a result, the level of heat release along with parasitic effects in junctions and conductors is less than the cooling effect.

Dagestan State Technical University, Makhachkala
Contact: S. M. Hajiyeva, kafedra.toe@mail.ru