I. A. Krasnov, M. A. Gavrik, E. P. Podolskaya, N. V. Krasnov
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METHOD ECD / ETD FRAGMENTATION OF THE PHOSPHOPEPTIDES IN MODERN TANDEM MASS SPECTROMETRY
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The paper presents features and the application of peptides fragmentation method in tandem mass spectrometry,
in particular fragmentation techniques CID, ECD and ETD, and their combinations. Mechanism of fragmentation, sample preparation methods and
sample introduction in relation to the method of ECD / ETD are analyzed. The possibility to use common proteomic databases for solving
phosphoproteomic tasks is shown. The conclusions about the effectiveness of CID / ETD fragmentation methods combination to expand amino
acid sequence restore capacity of the phosphopeptides are made.
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Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: I. A. Krasnov, krasnov.ilya@gmail.com
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p. 5-19
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E. N. Chernova1, Ya. V. Russkikh1, E. P. Podolskaya2,3, Z. A. Zhakovskaya1,
V. S. Tsarev1, G. I. Kukhareva1
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ANALYSIS OF CYANOTOXINES USING AN LTQ ORBITRAP (THERMO FINNIGAN):
THE OPTIMIZATION OF MASS-SPECTROMETRY METHOD
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Our current research includes the optimization of methods for detection, identification and occurring ñyanobacterial
toxines ("cyanotoxines"), which are the most ubiquitously found hazardous substances in surface waters. The detection limits of
the optimized method for cyanotoxines determination are lower than a provisional Guideline Value for cyanotoxines concentration in drinking
water provided by the World Health Organisation (1 μg/L).
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1Research Centre for Ecological Safety RAS, Saint-Petersburg
2Institute for Analytical Instrumentation RAS, Saint-Petersburg
3Institute of Toxicology of FMBA, Saint-Petersburg
Contact: E. N. Chernova, s3561389@yandex.ru
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p. 20-29
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E. N. Chernovà1, O. A. Keltsieva2, V. D. Gladilovich2,3, Ya. V. Russkikh1,
N. G. Sukhodolov2,4, À. À. Selutin4, V. A. Nikiforov1,
Z. A. Zhakovskaya1, E. P. Podolskaya2,3
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APPLICATION OF CHROMATO-MASS-SPECTROMETER OF HIGH RESOLUTION LTQ ORBITRAP FOR DETERMINATION
OF PERFLUORINATED ACIDS IN NATURAL WATER WITH THE TRADITIONAL SOLID-PHASE AND METALL-AFFINE
SORBENTS: METHOD DEVELOPMENT AND OPTIMIZATION
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Our current research includes the description of optimization procedure of the mass-spectrometry method for detection of perfluoroalkyl
acids in surface waters. The principle possibility of extracting perfluorooctansulfonate from water matrix by metal-affinity chromatography
on the new sorbents containing iron (III) ions was shown. The optimized method of chromato-mass-spectrometry analysis provides low limits
of detection below 1ng/L that allows the accurate quantification of perfluoroalkyl acids in water samples.
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1Research Centre for Ecological Safety RAS, Saint-Petersburg
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
3"Institute of Toxicology" of FMBA, Saint-Petersburg
4Saint Petersburg State University
Contact: E. N. Chernova, s3561389@yandex.ru
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p. 30-37
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Ya. A. Dubrovskiy1,2, E. A. Murashko1, M. Yu. Kombarova1,
E. P. Podolskaya2,3, V. N. Babakov1, N. V. Krasnov2, A. S. Radilov1
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IDENTIFICATION OF CYANIDE ADDUCT WITH HUMAN SERUM AL-BUMIN USING MASS-SPECTROMETRY
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MALDI mass spectrometry was used to identify cyanide adducts with human serum albumin. Peptide with MH+ 999.44 Da
was detected in the tryptic digestion of human serum albumin, incubated with cyanide (sodium and potassium salts). As a result SCN adduct at
Cys-34 are formed. The sequence Cys-Pro-Phe-Glu-Asp-His-Val-Lys was confirmed by HPLC-MS-MS analysis on ion trap.
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1Research Institute of Hygiene, Occupational Pathology and Human Ecology, Saint-Petersburg
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
3Institute of Toxicology FMBA of Russia, Saint-Petersburg
Contact: Ya. A. Dubrovskiy, dubrovskiy.ya@gmail.com
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p. 38-43
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I. A. Krasnov, D. E. Bobkov, M. Zaitseva, S. S. Prisyach, N. V. Krasnov
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THE DEVELOPMENT OF HPLC-ESI-MS(+) METHOD FOR URSODEOXYCHOLIC ACID ANALYSIS
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An analytical method for ursodeoxycholic acid has been developed based on high performance liquid chromatography separation coupled
to an electrospray (+) time-of-flight mass-spectrometer. Data about the change of UDCA concentration after intragastric injection
in rat serum depending on time are obtained.
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Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: I. A. Krasnov, krasnov.ilya@gmail.com
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p. 44-51
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Yu. K. Golikov1, N. K. Krasnova1, V. I. Nikolaev2,
K. V. Solovyev1
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ION’S SEPARATION IN THE STATIC FIELDS: QUADRUPOLE ELECTRIC AND HOMOGENEOUS MAGNETIC
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The behaviour of ion packet in the crossing static electromagnetic fields is investigated in the article.
Homogenous transverse magnetic field is applied to quadrupole analyser which works in the static mode. A number of finite trajectories
and the conditions for keeping ions in the canal of the device are determined by analytical methods. The initial conditions and parameters
of the fields are formulated when the system works as an ion’s trap or farvitron.
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1Saint-Petersburg State Polytechnic University
2"Scientific instruments", Saint-Petersburg
Contact: N. K. Krasnova, n.k.krasnova@mail.ru
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p. 52-60
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V. A. Elokhin1, Yu. K. Golikov2, K. V. Solovyev2
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PLANAR ELECTROSTATIC ION Z2 TRAPS
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The theory of the ion traps with ideal space-time focusing is developed. Ion traps discussed provide ideal ion
packet focusing along one of directions, time-focusing along the second coordinate and trapping along the third one.
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1"Scientific Instruments", Saint-Petersburg
2Saint-Petersburg State Polytechnical University
Contact: K. V. Solovyev, k-solovyev@mail.ru
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p. 61-67
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N. K. Krasnova1, Yu. K. Golikov1, V. A. Elokhin2,
V. I. Nikolaev2
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NEW DYNAMIC MASS SPECTROMETER WITH ELECTRIC IMPULSE
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Charge particle optics scheme of dynamic mass spectrometer is suggested. It is based on new ideas of mass analysis.
Ions of one energy are subjected to a number of short electric impulses in Δ-transformer, and as a result ions are distributed on energies.
Energy spectrum recorded by energy analyzer "Modification of arch" is strictly connected with mass spectrum. Different variants of charge
particle optics track are offered. Some estimations are done and perspective of dynamic mass spectrometry of new type is discussed.
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1Saint-Petersburg State Polytechnic University
2"Scientific instruments", Saint-Petersburg
Contact: N. K. Krasnova, n.k.krasnova@mail.ru
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p. 68-73
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O. A. Keltsieva1, V. D. Gladilovich1,2, E. P. Podolskaya1,2
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IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY (IMAC). PRINCIPLE AND APPLICATIONS
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This article reviews the basic principle of operation and the possibility of using immobilized metal affinity
chromatography which include cleaning of histidine-tagged proteins, metal-binding proteins and antibodies. The advantages and disadvantages
of this method and various sorbents are described.
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1Institute for Analytical Instrumentation of RAS, Saint-Petersburg
2Institute of Toxicology Federal Medico-Biological Agency of Russia, Saint-Petersburg
Contact: O. A. Keltsieva, keltcieva@gmail.com
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p. 74-85
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N. G. Sukhodolov1,2, N. S. Ivanov1, E. P. Podolskaya2,3
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NEW MATERIALS RECEIVED BY THE LANGMUIR—BLODGETT METHOD AND THEIR APPLICATION
IN NANOTECHNOLOGY AND INSTRUMENT (PART 1. HYBRID MATERIALS)
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Obtaining of Langmuir-Blodgett films containing various inorganic fragments, which represent self-assembled nano-objects was considered.
The possible and already realized ways of using these regular structures in various areas of modern materials science were presented.
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1Saint-Petersburg State University
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
3Institute of Toxicology FMBA of Russia, Saint-Petersburg
Contact: N. G. Sukhodolov, sng196505@mail.ru
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p. 86-105
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V. D. Gladilovich1,2, E. V. Shreyner1,4, Ya. A. Dubrovsky1,3, P. D. Kolonitsky4,
K. A. Krasnov2, E. V. Babina2, E. A. Murashko3, V. N. Babakov3,
O. A. Keltsieva1, I. A. Krasnov1, M. C. Anurov1,4, Ya. V. Russkih5,
E. N. Chernova5, Z. A. Zhakovskaya5, N. G. Suhodolov1,4,
A. A. Selyutin4, M. L. Alexandrova2, E. P. Podolskaya1,2
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STUDY OF THE SPECIFIC PROPERTIES OF MULTIMOLECULAR REGULAR SORBENT Fe(III)
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Now the problem of specific separation of a variety of substances from complex mixtures, including biological samples is very acute. One of the
methods that allows to solve such problems is the metal-affinity chromatography. Historically, it was used for the enrichment of phosphorus-containing
peptides, but it seems possible to use it for the enrichment of other classes of substances. In the paper the specific properties of a new regular
multimolecular sorbent based stearate iron (III) were discussed. It was shown that by using metal-affinity chromatography on RMMS Fe(III) such types
of compounds as organophosphorus (phosphorylated peptides, phosphonylated peptides, pesticides), fluorine acid (perfluorooctane sulfonic acid),
cyanobacteria toxins (microcystin-RR ) can be successfully identified and analyzed.
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1Institute for Analytical Instrumentation of RAS, Saint-Petersburg
2Institute of Toxicology of FMBA, Saint-Petersburg
3Research Institute of Hygiene, Occupational Pathology and Human Ecology, Saint-Petersburg
4Saint-Petersburg State University
5Research Centre for Ecological Safety RAS, Saint-Petersburg
Contact: E. V. Shreyner, shreyner.ekaterina@gmail.com
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p. 106-114
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A. A. Selyutin1, P. D. Kolonitskiy1, N. G. Sukhodolov1,2, E. V. Shreyner1,2,
N. V. Krasnov2, E. P. Podolskaya2,3
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SYNTHESIS AND CHARACTERIZATION OF NANOREGULAR SORBENTS BASED ON ZIRCONIUM OXIDE
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Application of a new method for the synthesis of ultrafine zirconium oxide from the aqueous solution with microwave
heat treatment yielded particles with dimensions less than at 10 nm. High specific surface area of the produced zirconia provided a good
selective sorption both of model objects (colorant Congo red) and real biological substances (casein).
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1Saint-Petersburg State University
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
3Institute of Toxicology of FMBA, Saint-Petersburg
Contact: A. A. Selyutin, selutin@inbox.ru
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p. 115-122
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N. G. Sukhodolov1,2, V. D. Gladilovich2,3, P. D. Kolonitskiy1, E. V. Shreyner1,2,
A. I. Yanklovich1, A. A. Selyutin1, N. V. Krasnov2,
E. P. Podolskaya2,3
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STUDY OF ELECTROKINETIC PROPERTIES OF REGULAR MULTIMOLECULAR SORBENT BASED ON TRIVALENT METALS STEARATES
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By the methods of streaming potential and microelectrophoresis electrokinetic properties of regular multimolecular sorbents
based on metallic stearates were investigated. It was shown that for two different types of objects such as Langmuir—Blodgett films transferred onto
a solid substrate and collapsed monolayers, dependences of the electrokinetic potential on pH of the electrolyte solution are practically identical,
which makes the collapsed monolayers of regular multimolecular structures similar to Langmuir—Blodgett films. The values of the isoelectric point for
a number of trivalent metal stearates were determined. Basing on these dependences conditions of metal-affinity chromatography sorbents on regular
multimolecular were proposed.
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1Saint-Petersburg State University
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
3Institute of Toxicology Federal Medico-Biological Agency of Russia, Saint-Petersburg
Contact: N. G. Sukhodolov, sng196505@mail.ru
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p. 123-129
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I. D. Kurdyukov1, E. A. Murashko1, Ya. A. Dubrovsky1,2, V. N. Babakov1, A. S. Radilov1
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APPLICATION OF HIGH-RESOLUTION MELTING ANALYSIS AND MALDI MINISEQUENCING FOR POLYMORPHISM GENOTYPING OF HUMAN PARAOXONASE-1 GENE
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High-resolution melting analysis (HRMA) and MALDI minisequencing were used to perform single nucleotide polymorphism (rs854560 and rs662) genotyping
of human paraoxonase-1 gene. The allelic frequencies (66.0 % for L-allele and 71.8 % for Q-allele) obtained from genotyping of 94 habitants
of Kirov region were similar to allelic frequencies of Europeans and Caucasian Americans. The experimental data confirmed the applicability of HRM
analysis and MALDI minisequencing for geno-typing of human paraoxonase-1 L55M and Q192R single nucleotide polymorphisms.
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1Research Institute of Hygiene, Occupational Pathology and Human Ecology (RIHOPHE), Saint-Petersburg
2Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Contact: I. D. Kurdyukov, splachnum@yandex.ru
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p. 130-135
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N. N. Stepanov, V. V. Kaminsky, A. A. Molodykh
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TEST OF STRAIN GAGES BASED ON SmS SEMICONDUCTOR THIN FILMS
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The authors propose a method for determining the gage factor of strain gages based on polycrystalline films of samarium
monosulphide (SmS) without loss of performance properties. We derive the relation between gage factor K and pressure coefficient of resistance under
hydrostatic compression B (barorezistor mode). It is show that the correlation between K and B is determined only by elastic constants of strain sensitive
layer of polycrystalline SmS and elastic constants of substrate on which it was formed.
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Ioffe Physical Technical Institute of the RAS, St. Petersburg
Contact: N. N. Stepanov, stnick@hotbox.ru
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p. 136-140
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CONTENTS |
| Contents Vol. 23 N1 >> |
